Cytotoxicity of 3D Printed Materials for Potential Dental Applications: An In Vitro Study

Aim of the Study: This study aims to evaluate cytotoxicity different stereolithographic 3D printed materials and polyetheretherketone for varying time intervals using MTT assay by application these intra-oral usage. Materials Methods: Three groups disc specimens (5 mm diameter, 2 thick) were manufactured either selective laser melting (PA6 nylon, fiber reinforced PA6 nylon) or milling (PEEK). The was tested culturing samples on human fibroblast cell lines prior assays (3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, a yellow tetrazole). completed at 3, 6, 10 days. Cell grown in normal medium without experimental material served as control. total number viability cells pre-incubated with fiber-reinforced PEEK comparable control samples. Differences between growth inhibitory effects below 0.05%. Results: Both nylon reduced proliferation fibroblasts up 6 days treatment. had better biocompatibility than nylon. are 3DPrinted that showed However, soaking made them safe cells. Conclusion: can be considered intraoral usage is biocompatible. increased in-vitro death days, suggesting they non-biocompatible beyond